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Abstract
The 6th International Symposium on Micro Total Analysis System (2002.11.3〜7;Nara)
Integrated microsystem of isothermal amplification of DNA and electrophoresis on a microfabricated plastic chip for detection of specific gene and analysis of genetic materials
Yukari Hataoka, Yoshinobu Baba
(Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation(JST)

Tsugunori Notomi,
(Eiken Chemical Co.,Ltd.)

Abstract
We have successfully realized the on-chip integration of isothermal polymerase chain reaction (PCR) and the subsequent analysis of the amplification products by electrophoresis. In our experiments, loop-mediated isothermal amplification (LAMP) has been adopted, which can not only avoid the thermal cycling, but also offer higher specific amplification than traditional PCR. After the amplification, the products could be either analyzed by the integrated microchip electrophoresis or directly observed by the naked eyes by adding some fluorescent dye like SYBR Green T. This integrated microsystem has proven extremely useful for the fast, sensitive, and simple detection of some specific sequences of DNA.

(1)Y. Baba et al.,Micro Total Analysis Systems 2002, Volume 1, 215-217

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