“LAMP” which stands for Loop-mediated Isothermal Amplification is characterized
by its use of four different primers specifically designed to recognize six distinct
regions on the target gene and its process being performed at a constant
temperature using a strand displacement reaction.
Amplification and detection of
target gene can be completed in a single step, by incubating the mixture of sample,
primers, DNA polymerase with strand displacement activity and substrates at a
constant temperature (in the region of 65°C).
It provides high amplification
efficiency, with DNA being amplified 109-1010 times in 15 - 60 minutes. Because of
its high specificity, the presence of the target gene sequence can easily be detected
just by judging presence of amplified products.
Eiken aims to apply the LAMP method to such fields as medicine, agriculture, food,
animal husbandry, environmental protection, and also develop and sale LAMP products, reagents and instruments, to detect bacterias, viruses and human genes.