 |
|
|
|
| The principle of LAMP method |
|
| The Loop Primers (either Loop Primer
B or Loop Primer F), containing sequences complementary to
the single stranded loop region (either between the B1 and
B2 regions, or between the F1 and F2 regions) on the 5' end
of the dumbbell-like structure, provide an increased number
of starting points for DNA synthesis for the LAMP method.
An example is shown in the figure where there is an amplified
product containing six loops. In the original LAMP method,
four of these loops would not be used, but through the use
of Loop Primers, all the single stranded loops can be used
as starting points for DNA synthesis.
|
|
|
|
|
|
|
Time saving by Loop Primer |
 |
|
|
|
The investigation on how Loop Primers
affect amplification time (original method: no Loop Primer;
rapid method: with Loop Primers) shows that the time required
for amplification with Loop Primers is one-third to one-half
of that without Loop Primer. With the use of Loop Primers,
amplification can be achieved within 30 minutes.
|
|
|
|
|
|
|
|
|
