Eiken Chemical Co,. Ltd.

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Loopamp  Verotoxin-producing Escherichia coli Detection Kit For the purpose of foods and environment inspection

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 Description

Amplification of nucleic acids is conducted by the LAMP method using the sequences related to Verotoxin (VT) gene as the primers. From whether the amplification occurred or not, the existence of Verotoxin-producing Escherichia coli can be determined.

By using Loopamp Realtime Turbidimeters to monitor the turbidity of white precipitates of magnesium pyrophosphate, the amplification by-product, the reaction can be detected.
About the instructions for using the kit, refer to the product information on this page.

Verotoxin-producing Escherichia coli Screening Kit

 Characteristics

1. Comparing with the conventional microorganism inspection methods in foods, the detection can be easily done with this kit in a shorter time (about 1 hr) from the samples of culture media.
2. Amplification and detection can be done in one single tube with the real-time turbidimeters. Electrophoresis is not required.
3. Sensitivity: 60 CFU(Colony Forming Unit) / test

 Contents of the kit

(1) Extraction Solution for Foods ( EX F)*1 1.8mL×3 tubes
(2) 1M Tris-HCl: pH7.0 ( Tris)*1 1.0mL×1 tube*2
(3) Reaction Mix. VT ( RM VT)*1 1.0mL×1 tube
(4) Bst DNA Polymerase ( Bst DNA Polymerase)*1 60 µL×1 tube
(5) Control DNA VT ( Cont VT)*1 0.1mL×1 tube

*1 The notation on each reagent tube is shown in ( ).
*2 Use for the extraction method of Japanese regulations.
* Use Loopamp Reaction Tube for the reactions.

 Unit, Storage, Expiration, Code No.

Unit Storage Expiration Code No.
48 tests -20°C 1 year LMP621

 More information

  Related documents
Product information (PDF)
 
  Related products
Loopamp Reaction Tube
Loopamp Realtime Turbidimeter

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