Eiken Chemical Co,. Ltd.

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Loopamp  Escherichia coli O157 Detection Kit For the purpose of foods and environment inspection

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 Description

Amplification of nucleic acids with this kit is conducted by the LAMP method using the sequences related to Escherichia coli serotype O157 as the primers. From whether the amplification occurred or not, the existence of Escherichia coli O157 can be determined.

By using Loopamp Realtime Turbidimeters to monitor the turbidity of white precipitates of magnesium pyrophosphate, the amplification by-product, the reaction can be detected.

Escherichia coli O157 Detection Kit

 Characteristics

1. The detection can be easily done with this kit in a shorter time (about 1 hr) from the samples of pre-culture media.
2. Amplification and detection can be done in one single tube with the real-time turbidimeters. Electrophoresis is not required.
3. Sensitivity: 60 CFU(Colony Forming Unit) / test

 Contents of the kit

(1) Extraction Solution for Foods ( EX F )*1 1.8mL×2 tubes
(2) 1M Tris-HCl (pH7.0) ( Tris )*1 1.0mL×1 tube
(3) 2×Reaction Mix. ( RM )*1 0.6mL×1 tube
(4) Bst DNA Polymerase ( Bst DNA Polymerase)*1 60µL×1 tube
(5) Distilled Water ( DW )*1 1.0mL×1 tube
(6) Primer Mix. O157 ( PM O157 )*1 0.12mL×1 tube
(7) Positive Control O157 ( PC O157 )*1 0.1mL×1 tube

*1 The notation on each reagent tube is shown in ( ).

* Use Loopamp Reaction Tube for the reactions.

 Unit, Storage, Expiration, Code No.

Unit Storage Expiration Code No.
48 tests -20°C 1 year LMP631

 More information

  Related documents
Product information (PDF)
 
  Related products
Loopamp Verotoxin-producing Escherichia coli Detection Kit
Loopamp Verotoxin Typing Kit
Loopamp Reaction Tube
Loopamp Realtime Turbidimeter

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