Eiken Chemical Co,. Ltd.

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Loopamp  Verotoxin Typing Kit For the purpose of foods and environment inspection

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 Description

Amplification of nucleic acids is conducted by the LAMP method using primers that can recognize the specific sequences of Verotoxin gene (VT1 and VT2) of enterohemorrhagic E. coli for the typing. From whether the amplification occurred or not, VT typing can be done.

By using Loopamp Realtime Turbidimeters to monitor the turbidity  of white precipitates of magnesium pyrophosphate, the amplification by-product, the reaction can be detected.
About the instructions for using the kit, refer to the product information on this page.

Verotoxin Typing Kit

 Characteristics

1. Comparing with the conventional microorganism inspection methods in foods, the typing can be easily done with this kit in a shorter time (about 1 hr).
2. Amplification and detection can be done in one single tube with the real-time turbidimeters. Electrophoresis is not required.
3. Sensitivity: 60 CFU(Colony Forming Unit) / test

 Contents of the kit

(1) Extraction Solution for Foods ( EX F)*1 1.8mL×1 tube
(2) Reaction Mix. VT1 ( RM V1)*1 0.5mL×1 tube
(3) Reaction Mix. VT2 ( RM V2)*1 0.5mL×1 tube
(4) Bst DNA Polymerase ( Bst DNA Polymerase)*1 60µL×1 tube
(5) Control DNA VT1 ( Cont V1)*1 0.1mL×1 tube
(6) Control DNA VT2 ( Cont V2)*1 0.1mL×1 tube

*1 The notation on each reagent tube is shown in ( ).

* Use Loopamp Reaction Tube for the reactions.

 Unit, Storage, Expiration, Code No.

Unit Storage Expiration Code No.
24 tests -20°C 1 year LMP641

 More information

  Related documents
Product information (PDF)
 
  Related products
Loopamp Reaction Tube
Loopamp Realtime Turbidimeter

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