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Detection methods - Electrophoresis detection

Visual detection Real-time detection Electrophoresis detection
 Example of DNA amplification (HBV)  
Example of DNA amplification (HBV)
This photograph presents the result of a 2% agarose gel electrophoresis after the amplification of HBV, which was amplified at 60°C for 1 hour. This result illustrates the typical electrophoresis pattern of LAMP amplified product, which is not a single band but a ladder pattern because LAMP method can form amplified products of various sizes consisting of alternately inverted repeats of the target sequence on the same strand.
The amplification was started with 6 copies in this example and the amplified product was authenticated by Ear I digestion. Finally, the sequences in these bands have been confirmed as to all come from the target gene.
 Example of RNA amplification (PSA mRNA)  
Example of RNA amplification (PSA mRNA)
This photograph presents the result of a 2% agarose gel electrophoresis after the amplification of PSA mRNA, which was amplified at 65°C for 1 hour.
This example illustrates the specific amplification of PSA mRNA in one PSA-expressing LNCaP cell mixed with one million PSA negative K562 cells. Similar with the HBV example above, the amplified product was authenticated by Sau 3A I digestion.
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