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Technical information
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Detection methods - Electrophoresis detection |
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Example of DNA amplification (HBV) |
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This photograph presents the
result of a 2% agarose gel electrophoresis after the
amplification of HBV, which was amplified at 60°C for
1 hour. This result illustrates the typical electrophoresis
pattern of LAMP amplified product, which is not a single
band but a ladder pattern because LAMP method can form
amplified products of various sizes consisting of alternately
inverted repeats of the target sequence on the same
strand.
The amplification was started with 6 copies in this
example and the amplified product was authenticated
by Ear I digestion. Finally, the sequences
in these bands have been confirmed as to all come from
the target gene. |
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Example of RNA amplification (PSA mRNA) |
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This photograph presents the
result of a 2% agarose gel electrophoresis after the
amplification of PSA mRNA, which was amplified at 65°C
for 1 hour.
This example illustrates the specific amplification
of PSA mRNA in one PSA-expressing LNCaP cell mixed with
one million PSA negative K562 cells. Similar with the
HBV example above, the amplified product was authenticated
by Sau 3A I digestion. |
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