Technical information  Detection methods - Visual detection Visual detection Real-time detection Electrophoresis detection  Visual detection by turbidity The turbidity of magnesium pyrophosphate, a by-product of the amplification reaction, is produced in proportion to the amount of amplified products. Since LAMP amplification can produce extremely large amount of amplified products, white turbidity can be visually observed. From this feature, the presence of turbidity can indicate the presence of target gene and visual detection can be achieved.  Visual detection by fluorescence   Calcein in Loopamp Fluorescent Detection Reagent was initially combined with manganese ions to achieve the quenching effect. The amplification generates the by-product, pyrophosphate ions, which will bind and remove manganese ions from calcein to irradiate fluorescence. The fluorescence is further intensified as calcein combines with magnesium ions. From this feature, the presence of fluorescence can indicate the presence of target gene and visual detection can be achieved. For reference If the tube containing the amplified products in the presence of fluorescent intercalating dye (ethidium bromide, etc.) is illuminated with a UV lamp, the fluorescence intensity increases. From this feature, the presence of fluorescence can indicate the presence of target gene and visual detection can be achieved.			LAMP standard procedures Detection methods SNPs typing Quantification method