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Detection methods - Visual detection

Visual detection Real-time detection Electrophoresis detection
 Visual detection by turbidity
Visual detection by turbidity
The turbidity of magnesium pyrophosphate, a by-product of the amplification reaction, is produced in proportion to the amount of amplified products. Since LAMP amplification can produce extremely large amount of amplified products, white turbidity can be visually observed. From this feature, the presence of turbidity can indicate the presence of target gene and visual detection can be achieved.
 Visual detection by fluorescence  
Visual detection by fluorescence
Calcein in Loopamp Fluorescent Detection Reagent was initially combined with manganese ions to achieve the quenching effect. The amplification generates the by-product, pyrophosphate ions, which will bind and remove manganese ions from calcein to irradiate fluorescence. The fluorescence is further intensified as calcein combines with magnesium ions. From this feature, the presence of fluorescence can indicate the presence of target gene and visual detection can be achieved.
For reference
For reference If the tube containing the amplified products in the presence of fluorescent intercalating dye (ethidium bromide, etc.) is illuminated with a UV lamp, the fluorescence intensity increases. From this feature, the presence of fluorescence can indicate the presence of target gene and visual detection can be achieved.
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