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Technical information
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SNPs typing - SNP typing on cloned DNA |

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CYP2C19 SNP typing using cloned DNA
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The figures show the SNP typing results (detected by
fluorescence) of CYP2C19 gene with Wild Type (WT) primers
(the upper figure) and with Mutant Type (MUT) primers
(the lower figure) respectively.
By using WT primers, only Wild Type template can be
amplified. Similarly, MUT primers only amplify Mutant
Type template. Therefore, SNP typing can be achieved
by detecting the presence of the amplified product.
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Validation
of the Loop-Mediated Isothermal Amplification
Method for Single Nucleotide Polymorphism Genotyping
with Whole Blood
Masaomi Iwasaki, Toshihiro Yonekawa, et al. Genome
Letters, Vol.2 (3) 119-126, 2003
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