Eiken Chemical Co,. Ltd.

Technical information 

SNPs typing - Procedures

Procedures SNP typing on cloned DNA SNP typing on human genome SNP typing using fluorescent intercalating dye
 Procedures
Genomic DNA
Primer (WT* or MUT**)
DNA polymerase
with strand displacement activity
dNTPs
Reaction Buffer
Fluorescent Detection Reagent
- closed system
- reaction at 60°C
detection  
By simply incubating genomic DNA and reagents, including Fluorescent Detection Reagent, at a constant temperature (60°C) for a fixed period of time, SNPs typing can be achieved by derterming whether amplification has taken place

* : Wild Type
** : Mutant Type

LAMP method allows the whole reaction process, including denaturing, to proceed at a constant temperature by incubating the reagents in a simple incubator. The presence of amplified product can be detected in a short time so as to provide a simple and rapid gene amplification method. Since 4 primers are designed to recognize 6 distinct regions on the target gene, only the target gene can be specifically amplified.
In addition, LAMP method has the characteristics of 1)no special reagents required, 2)no sophisticated temperature control device required, 3) the template can be simply detected through the presence of amplified product. Since it only requires simple equipments, cost effective gene test can be achieved. Both simple detection and real-time detection of the reaction are possible. Using Loop Primer can shorten amplification time by one third to one half.
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