For the purpose of foods and environment inspection
Loopamp Escherichia coli O157 Detection Kit
Description
Amplification of nucleic acids with this kit is conducted by the LAMP method using the sequences related to Escherichia coli serotype O157 as the primers. From whether the amplification occurred or not, the existence of Escherichia coli O157 can be determined.
By using Loopamp Realtime Turbidimeters to monitor the turbidity of white precipitates of magnesium pyrophosphate, the amplification by-product, the reaction can be detected.
Characteristics
- The detection can be easily done with this kit in a shorter time (about 1 hr) from the samples of pre-culture media.
- Amplification and detection can be done in one single tube with the real-time turbidimeters. Electrophoresis is not required.
- Sensitivity: 60 CFU (Colony Forming Unit) / test
Product Details
Product Name | Loopamp Escherichia coli O157 Detection Kit |
Unit | 48 tests |
Storage | - 20 ℃ |
Expiration | 1 year |
Code No. | LMP631 |
Contents | Extraction Solution for Foods (EX F) 1.8 mL x 2 1M Tris-HCl (pH7.0) (Tris ) 1.0 mL x 1 2 x Reaction Mix. (RM) 0.6 mL x 1 Bst DNA Polymerase (Bst DNA Polymerase) 60 µL x 1 Primer Mix. O157 (PM O157) 0.12 mL x 1 Positive Control O157 (PC O157) 0.1 mL x 1 |
- Use Loopamp Reaction Tube for the reactions.