For the purpose of foods and environment inspection
Loopamp Verotoxin-producing Escherichia coli Detection Kit
Description
Amplification of nucleic acids is conducted by the LAMP method using the sequences related to Verotoxin (VT) gene as the primers. From whether the amplification occurred or not, the existence of Verotoxin-producing Escherichia coli can be determined.
By using Loopamp Realtime Turbidimeters to monitor the turbidity of white precipitates of magnesium pyrophosphate, the amplification by-product, the reaction can be detected.
About the instructions for using the kit, refer to the product information on this page.
Characteristics
- Comparing with the conventional microorganism inspection methods in foods, the detection can be easily done with this kit in a shorter time (about 1 hr) from the samples of culture media.
- Amplification and detection can be done in one single tube with the real-time turbidimeters. Electrophoresis is not required.
- Sensitivity: 60 CFU (Colony Forming Unit) / test
Product Details
| Product Name | Loopamp Verotoxin-producing Escherichia coli Detection Kit |
| Unit | 48 tests |
| Storage | - 20 ℃ |
| Expiration | 1 year |
| Code No. | LMP621 |
| Contents | Extraction Solution for Foods (EX F) 1.8 mL x 3 1M Tris-HCl: pH7.0 (Tris) 1.0 mL x 1 Reaction Mix. VT (RM VT) 1.0 mL x 1 Bst DNA Polymerase (Bst DNA Polymerase) 60 µL x 1 Control DNA VT (Cont VT) 0.1 mL x 1 |
- Use for the extraction method of Japanese regulations.
- Use Loopamp Reaction Tube for the reactions.
