LAMP standard procedures

Standard procedures using LAMP method

Operation procedures of LAMP method

Reagents for LAMP method

• To amplify DNA :
  Four primers (FIP, F3, BIP, B3), DNA polymerase with strand displacement activity, substrates (deoxynucleotide triphosphate), and the reaction buffer are required.
• To amplify RNA :
  Add reverse transcriptase to the above reagents.

Procedure for the LAMP method

The procedure simply consists of incubating the template sample and the above reagents at a constant temperature between 60-65°C for 15 minutes to 1 hour, the amplification can be detected through the presence of amplified product.

In general, LAMP method allows the whole reaction process, including denaturing, proceeds at a constant temperature by incubating the reagents in a simple incubator. The presence of amplified product can be detected in a short time so as to provide a simple and rapid gene amplification method. Since 4 primers are designed to recognize 6 distinct regions on the target gene, only the target gene can be specifically amplified.
In addition, LAMP method has the characteristics of 1) no special reagent required, 2) no sophisticated temperature control device required, 3) the template can be simply detected through the presence of amplified product. Since it only requires simple equipment, cost effective genetic test can be achieved. Both simple detection and real-time detection of the reaction are possible. Using Loop Primer can shorten amplification time by one third to one half.